Uncapped duo Ander Iturraspe and Daniel Carvajal have both included in Vicente del Bosque’s 30-man Spain World Cup squad, along with Fernando Torres, but Real Madrid pair Alvaro Arbeloa and Isco have been omitted from the provisional panel.
Arbeloa was part of the Spain sides that have won back-to-back European Championships and the World Cup in the last six years.
Carvajal earned a spot after impressing following his return to Madrid last summer, while Athletic Bilbao midfielder Iturraspe is the other player in the squad yet to make a senior appearance for Spain.
Highly-rated Madrid playmaker Isco is among those who have missed out, as well as Tottenham striker Roberto Soldado, who has had a poor first season at White Hart Lane.
Chelsea striker Torres, who scored in the finals of both Euro 2008 and Euro 2012, has not had the best of campaigns either and has been absent from the last few Spain squads, but he has won a berth.
Aside from Torres, Del Bosque has included eight more Barclays Premier League players, including Liverpool goalkeeper Jose Reina, who has spent the season on loan at Napoli.
The others are Manchester City trio David Silva, Jesus Navas and Alvaro Negredo, Manchester United pair David De Gea and Juan Mata, Chelsea's Cesar Azpilicueta and Arsenal's Santi Cazorla.
The defending champions have included seven players from Barcelona and four each from Champions League finalists Real and Atletico Madrid.
Del Bosque will announce his final 23-man squad on 25 May.
Spain have a tough group in Brazil, with the Netherlands, the team they beat in the final four years ago, Chile and Australia alongside them in Group D.
Goalkeepers: Iker Casillas (Real Madrid), Jose Reina (Liverpool), David De Gea (Manchester United)
Defenders: Cesar Azpilicueta (Chelsea), Gerard Pique (Barcelona), Sergio Ramos (Real Madrid), Jordi Alba (Barcelona), Alberto Moreno (Sevilla), Javi Martinez (Bayern Munich), Raul Albiol (Napoli), Juanfran (Atletico Madrid), Daniel Carvajal (Real Madrid)
Midfielders: Xavi (Barcelona), Xabi Alonso (Real Madrid), Andres Iniesta (Barcelona), Koke (Atletico Madrid), Sergio Busquets (Barcelona), Santi Cazorla (Arsenal), Ander Iturraspe (Athletic Bilbao), Cesc Fabregas (Barcelona), Thiago Alcantara (Bayern Munich), Juan Mata (Manchester United), David Silva (Manchester City)
Forwards: Pedro (Barcelona), Jesus Navas (Manchester City), Diego Costa (Atletico Madrid), David Villa (Atletico Madrid), Fernando Torres (Chelsea), Alvaro Negredo (Manchester City), Fernando Llorente (Juventus)
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