Andres Iniesta's spectacular strike deep into stoppage time wrecked Chelsea's dreams of a second successive Champions League final against Manchester United amid chaotic scenes at Stamford Bridge.
Chelsea looked to have kept Barcelona at bay and sealed an all-Premier League clash in Rome on a night of nerve-jangling tension after Michael Essien's 20-yard blockbuster in the ninth minute.
But with Guus Hiddink's side hanging on - and fuelled by a sense of injustice after referee Tom Ovrebo rejected a succession of penalty appeals - Iniesta beat Petr Cech from the edge of the area to send Barcelona into the final on away goals.
It was a heartbreaking moment for Chelsea and one that was too much to bear for Didier Drogba, on the sidelines after being substituted with an injury.
There had already been angry scenes in the technical area after Barcelona's leveller, with Hiddink appearing to be buffeted on the touchline, but at the final whistle, Drogba marched on to the field to subject referee Ovrebo to a prolonged verbal tirade.
He rightly received a yellow card, but Drogba refused to be deterred by this punishment and continued to pursue the official while being held back.
It was a shameful conclusion to a dramatic encounter.
Chelsea looked on their way to the final after a superb defensive display that restricted Barcelona all night until Iniesta's deadly strike.
Drogba wasted a perfect opportunity to secure a second goal for Chelsea early in the second half, only to strike his finish against Barcelona keeper Victor Valdes.
But Chelsea's real bone of contention came from those penalty appeals, especially a first-half collision between Drogba and Eric Abidal, then a clear handball by Gerard Pique after the break.
Barcelona now face United in the final - but it was victory at a price with Dani Alves ruled out of the final after a booking and Abidal also missing after he was unluckily sent off for tangling with Nicolas Anelka.
Chelsea, meanwhile, must now gather their forces for the end of the Premier League campaign and the FA Cup final against Everton - but the glittering prize of the Champions League has eluded them once more.
Barcelona's attempt to secure a crucial away goal suffered a blow before kick-off when Thierry Henry was ruled out with a knee injury - while Chelsea included both Drogba and Anelka in their line-up.
The Catalan giants posed the first threat when Xavi's effort took two deflections before Jose Bosingwa cleared from almost on the goal-line.
And Chelsea took advantage of that escape to make the breakthrough after only nine minutes.
Frank Lampard's pass was half-cleared, and even though there seemed little danger, Essien met the loose ball with a thunderous left-foot volley that left Valdes stranded as it ripped high past him and into the net off the bar.
Barcelona's response was predictably measured as they refused to stray from their trademark passing approach, but it failed to reap any rewards as Chelsea keeper Cech remained well protected by his defenders.
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