An Italian navy officer has been arrested after being caught in the act of handing secret documents to a Russian military official, police say.
The men were stopped by military police on suspicion of serious crimes relating to espionage and state security.
The Russian, who reportedly worked at the Russian embassy in Italy, is expected to be expelled.
Russia's ambassador Sergey Razov has been summoned to the foreign ministry in Rome.
Italian Foreign Minister Luigi Di Maio said two Russian officials "involved in this very serious affair" were being expelled with immediate effect. The Kremlin said it hoped the "very positive and constructive nature of Russian-Italian relations will continue" but Russia's foreign ministry is expected to respond to the expulsions.
What happened in Rome
The carabinieri del Ros special operations group swooped on the men in Rome on Tuesday evening "during a clandestine meeting between the two," a police statement said.
They were "caught immediately after the transfer of a document by the Italian officer in exchange for a sum of money", it added.
Italian media reported that the exchange had taken place in a car park and involved €5,000 (£4,250; $5,900) in cash.
The Italian is described as being the captain of a frigate and the Russian officer's diplomatic status is currently being assessed.
Italy's Corriere website reports that papers seized in the naval officer's flat suggest he may have passed on Nato secrets, thereby placing other countries' national security at risk. According to the Ansa news agency, the documents involved military communications.
The Russian embassy confirmed the involvement of a military attaché official but said any further comment was inappropriate.
Kremlin spokesman Dmitry Peskov said "we don't have information about the reasons for or the circumstances of this detention". The foreign ministry in Moscow told Ansa that it regretted the expulsions of two embassy staff and would make a further announcement on possible steps in response.
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