Asante Kotoko, the last FA Cup holders will kick off the defence of their trophy with a home tie against Danbort FC in the Round of 64.
The Porcupine Warriors, who won the last Cup decade ago, were drawn against the Accra-based Division Two side following Friday's live draw for the Round of 64 of the MTN FA Cup.
Ties for the one-off games will be played on the weekends of 21-22 and 28-29 January.
Other intriguing ties see Hearts of Oak host Pure Joy and the all-premiership clashes - Berekum Chelsea take on AshantiGold, Dwarfs battle Aduana, Medeama play Real Tamale United.
All 16 Premier League sides were entered into the draw which also includes 17 Division Two sides and 31 clubs from the Division One League.
The competition has already recorded casualties but as it reaches the business stage, the possibility of having more upsets looms with some lower tier clubs drawing Premiership sides.
Round of 64 Draw
Golden Stars vRehoboth
Heart of Oak v Purejoy
Depotivo v Elmina Sharks
Berekum Arsenals v Ajax
Zaytuna v D'international
King Solomon v Mighty Jets
Heart of Lions v K.F.C. FC
Asante Kotoko v Danbort FC
Juventus vUnion Rovers
BA Utd v DC Utd
Tarkwa United v Yegola
Ebusua Dwarfs v Aduana Stars
Eleven Wise v Atebubu Esperance
Kintampo Zamalek v Shark FC
Windy Prof v Okwawu
Red bull v Cheetah FC
Bechem United v Yagbon Stars
Medeama SCv Real Tamale United
Starke FC v Jenak FC
Berekum Chelsea v AshantiGold
Universal Stars v Samartex
Unity FC v Bazooka
Great Olympics v Mighty Heroes
Inter-Allies v New Edubiase
King Faisal v Minestars
Liberty v Bofoakwa
St. Mirren v Metro Stars
No Weapon FC v All Stars FC
Shooting Stars v Dunkwa Utd
Wa All Stars v Istanbul FC
Nania FC v Mighty Youngsters
Berekum Friction v B.A. Stars
Source: Ghanafa.org
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