Burundi and Rwanda have been added to the "red list" of countries from where travel to the UK is banned.
The measure, aimed at stopping the spread of the South African variant, comes into force from 13:00GMT on Friday.
British, Irish and third country nationals with residence rights in the UK will be allowed to enter but must self-isolate for 10 days at home.
UK Transport Secretary Grant Shapps said passengers allowed to enter the country would need proof of a negative test and a completed passenger locator form before arrival, or could face two £500 fines.
From tomorrow (Friday 29 Jan at 1pm), we’re extending our travel ban with the United Arab Emirates, Burundi and Rwanda all added to the UK's red list. 1/3
— Rt Hon Grant Shapps MP (@grantshapps) January 28, 2021
Rwandan authorities last week imposed a 15-day lockdown in the capital, Kigali, to limit a second wave of coronavirus cases. The lockdown will end next Tuesday.
January has been the deadliest month for the East African country. The authorities say more than half of the total deaths were recorded this month, as well as one third of the total 13,885 cases.
Burundi early this month closed its land and water borders to contain the spread of the virus.
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